Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 81
Page 8-71
... Figure 8.16 . 6. Add 0.1 volume of 3 м sodium acetate ( pH 5.2 ) and 2 volumes of ethanol to fractions containing ... FIGURE 8.15 FIGURE 8.16 Ligation to Bacteriophage λ Arms Because of the Construction and Analysis of cDNA Libraries 8.71.
... Figure 8.16 . 6. Add 0.1 volume of 3 м sodium acetate ( pH 5.2 ) and 2 volumes of ethanol to fractions containing ... FIGURE 8.15 FIGURE 8.16 Ligation to Bacteriophage λ Arms Because of the Construction and Analysis of cDNA Libraries 8.71.
Page 15-14
... Figure 15.2 ) . ++ • Pancreatic DNAase I : In the presence of Mg ** , DNAase I behaves like an endonuclease that introduces nicks independently into each strand of double - stranded DNA . However , as discussed earlier , in the presence ...
... Figure 15.2 ) . ++ • Pancreatic DNAase I : In the presence of Mg ** , DNAase I behaves like an endonuclease that introduces nicks independently into each strand of double - stranded DNA . However , as discussed earlier , in the presence ...
Page 15-97
... Figure 15.9A ) , two separate sets of oligonucleotides are synthesized that are complementary to the opposite strands of the target DNA . One of these sets consists of a single species of oligonucleotide that is exactly complementary to ...
... Figure 15.9A ) , two separate sets of oligonucleotides are synthesized that are complementary to the opposite strands of the target DNA . One of these sets consists of a single species of oligonucleotide that is exactly complementary to ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector