Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 4
Page 13-16
... GATC GATC 11 Fi 41 1 << 10 18 01 10 1 • III III U W III GATC T ! 1 111 3 1 : 1 # !!! 1 M TIEK DI HUI > 3 # 1 Sequence is obtained from the end point of each deletion FIGURE 13.3A - primer 3 ↓ primer 2 primer 1 ← universal 13.16 DNA ...
... GATC GATC 11 Fi 41 1 << 10 18 01 10 1 • III III U W III GATC T ! 1 111 3 1 : 1 # !!! 1 M TIEK DI HUI > 3 # 1 Sequence is obtained from the end point of each deletion FIGURE 13.3A - primer 3 ↓ primer 2 primer 1 ← universal 13.16 DNA ...
Page 13-79
... GATC G 32p CGA 5 ' 3 ' GATC [ a - 32P ] dNTP 3 ' 5 ' Klenow fragment of E. coli DNA polymerase I 32 PN Isolate target DNA by cleavage with two restriction enzymes , only one of which generates a recessed 3 ' terminus Radiolabel recessed ...
... GATC G 32p CGA 5 ' 3 ' GATC [ a - 32P ] dNTP 3 ' 5 ' Klenow fragment of E. coli DNA polymerase I 32 PN Isolate target DNA by cleavage with two restriction enzymes , only one of which generates a recessed 3 ' terminus Radiolabel recessed ...
Page 15-111
... GATC- sequences . Mol . Gen. Genet . 195 : 126 . Matteucci , M.D. and M.H. Caruthers . 1981. Nucleotide chemistry . 4. Synthe- sis of deoxyoligonucleotides on a poly- mer support . J. Am . Chem . Soc . 103 : 3185 . Matteucci , M.D. and ...
... GATC- sequences . Mol . Gen. Genet . 195 : 126 . Matteucci , M.D. and M.H. Caruthers . 1981. Nucleotide chemistry . 4. Synthe- sis of deoxyoligonucleotides on a poly- mer support . J. Am . Chem . Soc . 103 : 3185 . Matteucci , M.D. and ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector