Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-82
... G.P. , K. Horiuchi , and N.D. Zinder . 1984. The functional origin of ... C.P. Hodgson , H.L. Moses , and M.J. Getz . 1982. Poly- adenylated RNA ... G.P. Thomas , and S.H. Hughes . 1983. 8.82 Construction and Analysis of cDNA Libraries ...
... G.P. , K. Horiuchi , and N.D. Zinder . 1984. The functional origin of ... C.P. Hodgson , H.L. Moses , and M.J. Getz . 1982. Poly- adenylated RNA ... G.P. Thomas , and S.H. Hughes . 1983. 8.82 Construction and Analysis of cDNA Libraries ...
Page 10-51
... Cp Cp Tp Ap Gp 5 ' [ a - 32P ] dGTP GOH 3 ' To Ap Go 5 ' G & G OH 31 3 ' Blunt - ended DNA fragments are labeled by replacement of the nucleotide present at the 3 ' - hydroxyl terminus . • 5 ' To C p Ρ [ a - 32P ] dGTP T 5 ' · AG OH 31 ...
... Cp Cp Tp Ap Gp 5 ' [ a - 32P ] dGTP GOH 3 ' To Ap Go 5 ' G & G OH 31 3 ' Blunt - ended DNA fragments are labeled by replacement of the nucleotide present at the 3 ' - hydroxyl terminus . • 5 ' To C p Ρ [ a - 32P ] dGTP T 5 ' · AG OH 31 ...
Page 10-53
... Cp Tp Ap Gp 5 ' [ a - 32P ] dGTP 5 ' DA PA 3. A * G [ a - 32P ] dATP 3 ' GOH 3 Cp Cp Tp Ap Gp 5 ' 5 ' PA PA PT PT PC G * G OH 3 ' 3 ' HO G Cp Cp Tp Ap Gp 5 ' грар LABELING THE 3 ' TERMINI OF DOUBLE - STRANDED DNA Preparation of ...
... Cp Tp Ap Gp 5 ' [ a - 32P ] dGTP 5 ' DA PA 3. A * G [ a - 32P ] dATP 3 ' GOH 3 Cp Cp Tp Ap Gp 5 ' 5 ' PA PA PT PT PC G * G OH 3 ' 3 ' HO G Cp Cp Tp Ap Gp 5 ' грар LABELING THE 3 ' TERMINI OF DOUBLE - STRANDED DNA Preparation of ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector