Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 16
Page 8-18
... HindIII Digest with HindIII Pvull Hindill CCCCC RNAase H. TTTTTT Digest with Hpal Purify large fragment of DNA by gel electrophoresis and chromatography on oligo ( dA ) - cellulose Hpal Pvull Hindill Digest with Kpnl Kpnl Pvull Add oligo ...
... HindIII Digest with HindIII Pvull Hindill CCCCC RNAase H. TTTTTT Digest with Hpal Purify large fragment of DNA by gel electrophoresis and chromatography on oligo ( dA ) - cellulose Hpal Pvull Hindill Digest with Kpnl Kpnl Pvull Add oligo ...
Page 8-39
... ( HindIII / BamHI / EcoRI ) at codons 5-10 of the lacZ gene ( see map of λORF8 in Chapter 2 , page 2.50 ) . Insertion ... HindIII . Because M.HindIII methylase is not commercially available , the cDNA is methylated with M.BamHI methylase ...
... ( HindIII / BamHI / EcoRI ) at codons 5-10 of the lacZ gene ( see map of λORF8 in Chapter 2 , page 2.50 ) . Insertion ... HindIII . Because M.HindIII methylase is not commercially available , the cDNA is methylated with M.BamHI methylase ...
Page 8-41
... HindIII site is formed by fusion of the poly ( dT : dA ) tract at the 3 ' terminus of the cDNA to the first linker ( see Figure 8.13 ) . Addition of linkers to the 5 ' terminus of the cDNA does not usually create a new restriction site ...
... HindIII site is formed by fusion of the poly ( dT : dA ) tract at the 3 ' terminus of the cDNA to the first linker ( see Figure 8.13 ) . Addition of linkers to the 5 ' terminus of the cDNA does not usually create a new restriction site ...
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agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector