Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 71
Page 10-22
... reaction by tapping the side of the tube . Incubate the reaction for 30 minutes at room temperature . 6. Transfer 0.5 μl of the reaction to a fresh microfuge tube containing 20 μl of 0.5 M EDTA ( pH 8.0 ) . Store the tube on ice . 7 ...
... reaction by tapping the side of the tube . Incubate the reaction for 30 minutes at room temperature . 6. Transfer 0.5 μl of the reaction to a fresh microfuge tube containing 20 μl of 0.5 M EDTA ( pH 8.0 ) . Store the tube on ice . 7 ...
Page 10-55
... incubate for the appropriate time . If the desired restriction enzyme does not function in bacteriophage T4 DNA ... reaction for 5 minutes at 37 ° C . 7. Add 1 μl of a 2 mm solution of the unlabeled fourth dNTP . Continue the incubation for a ...
... incubate for the appropriate time . If the desired restriction enzyme does not function in bacteriophage T4 DNA ... reaction for 5 minutes at 37 ° C . 7. Add 1 μl of a 2 mm solution of the unlabeled fourth dNTP . Continue the incubation for a ...
Page 14-26
... incubate the reaction mixture in a boiling- water bath for 2 minutes . 3. Transfer the tube to a microfuge , and centrifuge at 12,000g for 5 seconds at room temperature . 4. Immediately transfer the tube to a water bath set at 37 ° C .
... incubate the reaction mixture in a boiling- water bath for 2 minutes . 3. Transfer the tube to a microfuge , and centrifuge at 12,000g for 5 seconds at room temperature . 4. Immediately transfer the tube to a water bath set at 37 ° C .
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector