Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-40
... FRAGMENT OF E. coli DNA POLYMERASE I In some cases ( e.g. , when using oligonucleotides as probes in Southern hybridizations ) , it is important to radiolabel to the highest possible ... KLENOW FRAGMENT OF E coli DNA POLYMERASE I 11 40.
... FRAGMENT OF E. coli DNA POLYMERASE I In some cases ( e.g. , when using oligonucleotides as probes in Southern hybridizations ) , it is important to radiolabel to the highest possible ... KLENOW FRAGMENT OF E coli DNA POLYMERASE I 11 40.
Page 13-79
... Klenow fragment of E. coli DNA polymerase I 32 PN Isolate target DNA by cleavage with two restriction enzymes , only one of which generates a recessed 3 ' terminus Radiolabel recessed terminus with the appropriate [ a - 32P ] dNTP and ...
... Klenow fragment of E. coli DNA polymerase I 32 PN Isolate target DNA by cleavage with two restriction enzymes , only one of which generates a recessed 3 ' terminus Radiolabel recessed terminus with the appropriate [ a - 32P ] dNTP and ...
Page 15-64
... Klenow fragment of E. coli DNA Store the PE3 mixture on ice until it is needed . 10 x PE2 buffer 200 mM Tris Cl ( pH 7.5 ) 100 mM MgCl2 100 mм dithiothreitol For definition of Weiss unit , see page 15.30 . Any of several different DNA ...
... Klenow fragment of E. coli DNA Store the PE3 mixture on ice until it is needed . 10 x PE2 buffer 200 mM Tris Cl ( pH 7.5 ) 100 mM MgCl2 100 mм dithiothreitol For definition of Weiss unit , see page 15.30 . Any of several different DNA ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector