Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 52
Page 12-38
... MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes . 4. To three of the tubes , add 1 × 107 , 5 × 107 , or 2 ...
... MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes . 4. To three of the tubes , add 1 × 107 , 5 × 107 , or 2 ...
Page 14-20
... MgCl2 in the amplification buffer needs to be supplemented with additional MgCl2 in the reverse transcriptase reaction mixture because reverse transcriptase requires higher concentrations of divalent cations than does Taq DNA polymerase ...
... MgCl2 in the amplification buffer needs to be supplemented with additional MgCl2 in the reverse transcriptase reaction mixture because reverse transcriptase requires higher concentrations of divalent cations than does Taq DNA polymerase ...
Page 15-29
... MgCl2 1 mм dithiothreitol The use of two different DNA polymerases in the repair reaction results in an approximately threefold increase in recovery of mutants . 11. Add 70 μl of TE ( pH 7.6 ) , and then extract the sample once with an ...
... MgCl2 1 mм dithiothreitol The use of two different DNA polymerases in the repair reaction results in an approximately threefold increase in recovery of mutants . 11. Add 70 μl of TE ( pH 7.6 ) , and then extract the sample once with an ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector