Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-54
... acetate ( pH 8.0 ) 0.1-2 με 2 μl 0.66 м potassium acetate 0.1 м magnesium acetate 5 mm dithiothreitol 1 mg / ml bovine serum albumin ( Fraction V ; Sigma ) The 10 stock should be divided into small aliquots and stored frozen at -20 ° C ...
... acetate ( pH 8.0 ) 0.1-2 με 2 μl 0.66 м potassium acetate 0.1 м magnesium acetate 5 mm dithiothreitol 1 mg / ml bovine serum albumin ( Fraction V ; Sigma ) The 10 stock should be divided into small aliquots and stored frozen at -20 ° C ...
Page 13-84
... acetate ( pH 7.0 ) 1 M ẞ - mercaptoethanol 250 μg / ml yeast tRNA Chill to -20 ° C . 95 % ( Eastman Kodak catalog no . 902 ) . Hydrazine should be stored in small aliquots in tightly capped microfuge tubes at -20 ° C . 0.3 M sodium acetate ...
... acetate ( pH 7.0 ) 1 M ẞ - mercaptoethanol 250 μg / ml yeast tRNA Chill to -20 ° C . 95 % ( Eastman Kodak catalog no . 902 ) . Hydrazine should be stored in small aliquots in tightly capped microfuge tubes at -20 ° C . 0.3 M sodium acetate ...
Page 13-98
... acetate ) , after which piperidine breaks the DNA at guanines . Residual sodium acetate from the second ethanol precipitation buffers piperidine , leading to incomplete phosphate elimina- tion reactions , which require a high pH . The 3 ...
... acetate ) , after which piperidine breaks the DNA at guanines . Residual sodium acetate from the second ethanol precipitation buffers piperidine , leading to incomplete phosphate elimina- tion reactions , which require a high pH . The 3 ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector