Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 23
Page 11-23
... Acrylamide stock acrylamide ( DNA - sequencing grade ) N , N ' - methylenebisacrylamide H2O to 250 ml 190 g 10 g Dissolve the chemicals by heating the solution to 37 ° C . Adjust the volume of the solution to 500 ml . Filter the ...
... Acrylamide stock acrylamide ( DNA - sequencing grade ) N , N ' - methylenebisacrylamide H2O to 250 ml 190 g 10 g Dissolve the chemicals by heating the solution to 37 ° C . Adjust the volume of the solution to 500 ml . Filter the ...
Page 11-26
... acrylamide solution from the syringe , filling the space almost to the top . If the plates were clean , there should be no trapped air bubbles , and if they were sealed well , no leaks . If air bubbles form , empty the gel mold and ...
... acrylamide solution from the syringe , filling the space almost to the top . If the plates were clean , there should be no trapped air bubbles , and if they were sealed well , no leaks . If air bubbles form , empty the gel mold and ...
Page 13-48
... Acrylamide / urea top solution 40 % acrylamide solution 5x TBE urea ( ultrapure ) 75 ml 50 ml 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 ...
... Acrylamide / urea top solution 40 % acrylamide solution 5x TBE urea ( ultrapure ) 75 ml 50 ml 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector