Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 23
Page xxx
... AGAR OR AGAROSE A.4 STORAGE MEDIA A.5 Stab Cultures A.5 Cultures Containing Glycerol A.5 BACTERIAL CULTURES GROWING IN LIQUID MEDIA A.5 BACTERIAL CULTURES GROWING ON AGAR PLATES A.5 ANTIBIOTICS A.6 SOLUTIONS FOR WORKING WITH ...
... AGAR OR AGAROSE A.4 STORAGE MEDIA A.5 Stab Cultures A.5 Cultures Containing Glycerol A.5 BACTERIAL CULTURES GROWING IN LIQUID MEDIA A.5 BACTERIAL CULTURES GROWING ON AGAR PLATES A.5 ANTIBIOTICS A.6 SOLUTIONS FOR WORKING WITH ...
Page 9-30
... agar or agarose with each aliquot of infected bacteria and spread on a freshly poured 150 - mm plate of bottom agar . Alternatively , as many as 450,000 bacteriophages may be mixed with 14 ml of bacteria and plated in 75 ml of top agar ...
... agar or agarose with each aliquot of infected bacteria and spread on a freshly poured 150 - mm plate of bottom agar . Alternatively , as many as 450,000 bacteriophages may be mixed with 14 ml of bacteria and plated in 75 ml of top agar ...
Page 12-20
... agar from this area using the large end of a pasteur pipette . Transfer the plug to 1 ml of SM containing 2 drops of chloroform . e . Keep the sheet of Saran Wrap , which provides a permanent record of the locations of the positive ...
... agar from this area using the large end of a pasteur pipette . Transfer the plug to 1 ml of SM containing 2 drops of chloroform . e . Keep the sheet of Saran Wrap , which provides a permanent record of the locations of the positive ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector