Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiii
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE CONCENTRATION 6.4 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE CONCENTRATION 6.4 ...
Page 9-34
A Laboratory Manual Joseph Sambrook, Tom Maniatis. TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS There are three methods to transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS There are three methods to transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) ...
Page 9-41
... AGAROSE GEL TO TWO NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the ...
... AGAROSE GEL TO TWO NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the ...
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agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector