Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-57
... aliquots at -70 ° C . Make a stock solution in water . Store in small aliquots at -20 ° C . Use strains C600 ( BNN93 ) for growth and BNN102 ( C600hflA ) for screening of cDNA libraries constructed in bacteriophage Agt10 . Use strain ...
... aliquots at -70 ° C . Make a stock solution in water . Store in small aliquots at -20 ° C . Use strains C600 ( BNN93 ) for growth and BNN102 ( C600hflA ) for screening of cDNA libraries constructed in bacteriophage Agt10 . Use strain ...
Page 8-66
... aliquots ( 2 μl each ) from the large - scale reaction and place in small microfuge tubes . Number these aliquots 3 and 4 . 5. To all four aliquots , add 100 ng of a plasmid such as Xf3 or pBR322 that has been digested to completion ...
... aliquots ( 2 μl each ) from the large - scale reaction and place in small microfuge tubes . Number these aliquots 3 and 4 . 5. To all four aliquots , add 100 ng of a plasmid such as Xf3 or pBR322 that has been digested to completion ...
Page 10-7
... aliquots and store them at -20 ° C . 10 x Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 100 mм dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) ( optional ) Divide the stock solution into small aliquots and ...
... aliquots and store them at -20 ° C . 10 x Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 100 mм dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) ( optional ) Divide the stock solution into small aliquots and ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector