Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-27
... allow the sequences at the 5 ' terminus of the mRNA ( which are often lost during conventional cloning procedures ) to be cloned . Its major disadvantage , however , is that it is at least ten times less efficient than double - stranded ...
... allow the sequences at the 5 ' terminus of the mRNA ( which are often lost during conventional cloning procedures ) to be cloned . Its major disadvantage , however , is that it is at least ten times less efficient than double - stranded ...
Page 8-32
... allow DNA : RNA hybrids to be removed by equilibrium centrifugation in CsCl gradients containing guanidine HCl ... allows single - stranded DNA of the desired size to be cloned and , by the use of primer - adapters , it avoids digesting ...
... allow DNA : RNA hybrids to be removed by equilibrium centrifugation in CsCl gradients containing guanidine HCl ... allows single - stranded DNA of the desired size to be cloned and , by the use of primer - adapters , it avoids digesting ...
Page 8-70
... allow the cDNA to enter the gel matrix . Wash the microfuge tube used to store the cDNA with 50 μl of TE ( pH 7.6 ) , and apply this to the column . Fill the bubble tubing with TE ( pH 7.6 ) containing 0.1 M NaCl . Important : Do not allow ...
... allow the cDNA to enter the gel matrix . Wash the microfuge tube used to store the cDNA with 50 μl of TE ( pH 7.6 ) , and apply this to the column . Fill the bubble tubing with TE ( pH 7.6 ) containing 0.1 M NaCl . Important : Do not allow ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector