Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 8
Page 9-8
... amber mutations in the arms . Amber mutations are carried in essential genes in many currently used vectors ( or their derivatives ) . This allows genomic DNA libraries to be screened for sequences of interest by the elegant ...
... amber mutations in the arms . Amber mutations are carried in essential genes in many currently used vectors ( or their derivatives ) . This allows genomic DNA libraries to be screened for sequences of interest by the elegant ...
Page 15-51
... mutations on the single - stranded genome of the small bac- teriophage X174 ... amber mutation , " marker rescue " was observed , i.e. , bacteriophages ... Mutagenesis of Cloned DNA 15.51 Oligonucleotide-mediated Mutagenesis 15 51.
... mutations on the single - stranded genome of the small bac- teriophage X174 ... amber mutation , " marker rescue " was observed , i.e. , bacteriophages ... Mutagenesis of Cloned DNA 15.51 Oligonucleotide-mediated Mutagenesis 15 51.
Page 1-21
... mutagenesis , 15.74-15.79 lac5 , 2.16 lacla L -encoding plasmid pMC9 , 2.57 genetic marker in E. coli host strain ... amber mutations , 9.8 ligation to fragments of foreign DNA , 2.94 purification , 2.85-2.89 assembly pathway , 2.7 ...
... mutagenesis , 15.74-15.79 lac5 , 2.16 lacla L -encoding plasmid pMC9 , 2.57 genetic marker in E. coli host strain ... amber mutations , 9.8 ligation to fragments of foreign DNA , 2.94 purification , 2.85-2.89 assembly pathway , 2.7 ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector