Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 91
Page 8-73
... amount of bacteriophage A arms is ligated to varying amounts of cDNA . The aim is to determine the amount of cDNA that yields at least 5 × 106 recombinant bacteriophages ( i.e. , a mammalian cDNA library of reasonable size ) . The ...
... amount of bacteriophage A arms is ligated to varying amounts of cDNA . The aim is to determine the amount of cDNA that yields at least 5 × 106 recombinant bacteriophages ( i.e. , a mammalian cDNA library of reasonable size ) . The ...
Page 10-9
... amount of the unlabeled dNTP . Most commercial suppliers sell [ a - 32P ] dNTPs in a concentrated aqueous solution ... amount of DNAase I in the reaction . The aim is to establish conditions that will result in incorporation of about 30 ...
... amount of the unlabeled dNTP . Most commercial suppliers sell [ a - 32P ] dNTPs in a concentrated aqueous solution ... amount of DNAase I in the reaction . The aim is to establish conditions that will result in incorporation of about 30 ...
Page 14-31
... amount of radioactivity in them in a liquid scintillation counter . Calculate the relative amounts of the two radiolabeled DNAs in each of the polymer- ase chain reactions . Remember to correct the amount of radioactivity to allow for ...
... amount of radioactivity in them in a liquid scintillation counter . Calculate the relative amounts of the two radiolabeled DNAs in each of the polymer- ase chain reactions . Remember to correct the amount of radioactivity to allow for ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector