Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxx
... BACTERIAL CULTURES GROWING IN LIQUID MEDIA A.5 BACTERIAL CULTURES GROWING ON AGAR PLATES A.5 ANTIBIOTICS A.6 SOLUTIONS FOR WORKING WITH BACTERIOPHAGE X A.7 Maltose A.7 SM A.7 TM A.7 A Diluent A.8 BACTERIAL STRAIN LIST A.9 Appendix B ...
... BACTERIAL CULTURES GROWING IN LIQUID MEDIA A.5 BACTERIAL CULTURES GROWING ON AGAR PLATES A.5 ANTIBIOTICS A.6 SOLUTIONS FOR WORKING WITH BACTERIOPHAGE X A.7 Maltose A.7 SM A.7 TM A.7 A Diluent A.8 BACTERIAL STRAIN LIST A.9 Appendix B ...
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... bacterial plates used to select transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at -20 ° C . 11. From the numbers of colonies obtained ...
... bacterial plates used to select transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at -20 ° C . 11. From the numbers of colonies obtained ...
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... bacterial conjugation , 1.5 open reading frame vectors for bacterial expression , 17.4 , 17.7 PMC9 , genetic marker in bacteriophage A host strain , 2.57 recombinant , detection in bacterial colonies a - complementation of defective ß ...
... bacterial conjugation , 1.5 open reading frame vectors for bacterial expression , 17.4 , 17.7 PMC9 , genetic marker in bacteriophage A host strain , 2.57 recombinant , detection in bacterial colonies a - complementation of defective ß ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector