Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xi
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Plating Bacteriophage M13 4.22 Picking Bacteriophage M13 Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Plating Bacteriophage M13 4.22 Picking Bacteriophage M13 Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 ...
Page 15-52
... bacteriophage M13 vector 2. Preparation of single - stranded DNA from the recombinant bacteriophage M13 3. Design and synthesis of mutagenic oligonucleotides 4. Hybridization of mutagenic oligonucleotides to the target DNA 5. Extension ...
... bacteriophage M13 vector 2. Preparation of single - stranded DNA from the recombinant bacteriophage M13 3. Design and synthesis of mutagenic oligonucleotides 4. Hybridization of mutagenic oligonucleotides to the target DNA 5. Extension ...
Page 15-63
... bacteriophage M13 vector ( usually bacteriophage M13mp18 or M13mp19 ) . Prepare single - stranded template DNA from a plaque generated by the recombinant bacteriophage . Methods for cloning into bacteriophage M13 vectors and for ...
... bacteriophage M13 vector ( usually bacteriophage M13mp18 or M13mp19 ) . Prepare single - stranded template DNA from a plaque generated by the recombinant bacteriophage . Methods for cloning into bacteriophage M13 vectors and for ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector