Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 77
Page 8-56
... Bacteriophage λ arms Bacteriophage T4 DNA ligase Required only if self - priming of second - strand cDNA synthesis in un- acceptably high ( see note to step 5g on page 8.65 ) . Preparations of actinomycin D supplied by pharmaceutical ...
... Bacteriophage λ arms Bacteriophage T4 DNA ligase Required only if self - priming of second - strand cDNA synthesis in un- acceptably high ( see note to step 5g on page 8.65 ) . Preparations of actinomycin D supplied by pharmaceutical ...
Page 10-54
... DNA WITH BACTERIOPHAGE T4 DNA POLYMERASE Rapid End - labeling of DNA Bacteriophage T4 DNA polymerase , like the Klenow fragment of E. coli DNA polymerase I , can be used to label flush or recessed 3 ' termini of DNA . However , ...
... DNA WITH BACTERIOPHAGE T4 DNA POLYMERASE Rapid End - labeling of DNA Bacteriophage T4 DNA polymerase , like the Klenow fragment of E. coli DNA polymerase I , can be used to label flush or recessed 3 ' termini of DNA . However , ...
Page 10-56
... bacteriophage T4 DNA polymerase first digests double - stranded DNA to produce molecules with recessed 3 ' termini . On subsequent addition of radiolabeled dNTPs , the partially digested DNA molecules serve as primer : templates that ...
... bacteriophage T4 DNA polymerase first digests double - stranded DNA to produce molecules with recessed 3 ' termini . On subsequent addition of radiolabeled dNTPs , the partially digested DNA molecules serve as primer : templates that ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector