Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 84
Page 11-17
... bases , a “ neutral ” base can be used that pairs adequately with all four conventional bases . The deleterious effect of mismatching is greatly reduced because the base pairs formed between the neutral base and its conventional ...
... bases , a “ neutral ” base can be used that pairs adequately with all four conventional bases . The deleterious effect of mismatching is greatly reduced because the base pairs formed between the neutral base and its conventional ...
Page 13-11
... base or type of base . This generates five populations of radiolabeled molecules that extend from a common point ( the radiolabeled terminus ) to the site of chemical cleavage . Each population consists of a mixture of molecules whose ...
... base or type of base . This generates five populations of radiolabeled molecules that extend from a common point ( the radiolabeled terminus ) to the site of chemical cleavage . Each population consists of a mixture of molecules whose ...
Page 15-108
... base is thus incorporated at a high frequency , and the remainder of the gap is then filled in a second polymerization reaction carried out in the presence of all four of the normal dNTPs . All types of base substitutions have been ...
... base is thus incorporated at a high frequency , and the remainder of the gap is then filled in a second polymerization reaction carried out in the presence of all four of the normal dNTPs . All types of base substitutions have been ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector