Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-7
... cDNA library is N = In ( 1 - P ) In ( 1-1 / n ) where N = the number of clones required , P = the probability ... libraries that contain several million independent cDNA clones . During the last few years , the efficiency with which cDNA ...
... cDNA library is N = In ( 1 - P ) In ( 1-1 / n ) where N = the number of clones required , P = the probability ... libraries that contain several million independent cDNA clones . During the last few years , the efficiency with which cDNA ...
Page 8-46
... cDNA libraries for clones of interest : • Nucleic acid hybridization • Immunological detection of specific antigens ... cDNA sequences , whereas antibodies will react only with a subset of these clones ( in some cases one in six at best ) ...
... cDNA libraries for clones of interest : • Nucleic acid hybridization • Immunological detection of specific antigens ... cDNA sequences , whereas antibodies will react only with a subset of these clones ( in some cases one in six at best ) ...
Page 8-53
... cDNA libraries in bacteriophage A vectors . The scheme is based on the procedures of Gubler and Hoffman ( 1983 ) and Huynh et al . ( 1985 ) and has become the standard procedure used to ... cDNA Libraries 8.53 Protocols for cDNA Cloning 8.
... cDNA libraries in bacteriophage A vectors . The scheme is based on the procedures of Gubler and Hoffman ( 1983 ) and Huynh et al . ( 1985 ) and has become the standard procedure used to ... cDNA Libraries 8.53 Protocols for cDNA Cloning 8.
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector