Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-46
... carrying the DNA to a source of ultraviolet irradiation ( 254 nm ) . Caution : Ultraviolet radiation is dangerous , particularly to the eyes . To minimize exposure , make sure that the ultraviolet light source is adequately shielded and ...
... carrying the DNA to a source of ultraviolet irradiation ( 254 nm ) . Caution : Ultraviolet radiation is dangerous , particularly to the eyes . To minimize exposure , make sure that the ultraviolet light source is adequately shielded and ...
Page 15-43
... with BglII , and purify the 1.4 - kb fragment carrying the kan ' gene by electrophoresis through a 0.8 % agarose gel . Ligation of linearized plasmid DNA to the fragment carrying the Site - directed Mutagenesis of Cloned DNA 15.43 37.
... with BglII , and purify the 1.4 - kb fragment carrying the kan ' gene by electrophoresis through a 0.8 % agarose gel . Ligation of linearized plasmid DNA to the fragment carrying the Site - directed Mutagenesis of Cloned DNA 15.43 37.
Page 15-44
... carrying the kan ' gene 1. Ligate the linearized plasmid DNA ( step 6 , page 15.42 ) to the fragment carrying the kan ' gene ( step 7 , page 15.43 ) . Because the efficiency with which the two fragments will ligate to each other is ...
... carrying the kan ' gene 1. Ligate the linearized plasmid DNA ( step 6 , page 15.42 ) to the fragment carrying the kan ' gene ( step 7 , page 15.43 ) . Because the efficiency with which the two fragments will ligate to each other is ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector