Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 95
Page xxvi
... Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES 16.3 FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and Amplification ...
... Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES 16.3 FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and Amplification ...
Page 9-16
... cell samples Cells growing in monolayers : Wash the monolayers twice with ice - cold Tris - buffered saline ( TBS ; see Appendix B ) . Using a policeman , scrape the cells into approximately 0.5 ml of TBS . Transfer the cell suspension ...
... cell samples Cells growing in monolayers : Wash the monolayers twice with ice - cold Tris - buffered saline ( TBS ; see Appendix B ) . Using a policeman , scrape the cells into approximately 0.5 ml of TBS . Transfer the cell suspension ...
Page 10-38
... cells . The identifi- cation of cDNA clones may be accomplished by a number of methods , all of which are capable of detecting differences in gene expression between closely related types of cells . This section presents methods to ...
... cells . The identifi- cation of cDNA clones may be accomplished by a number of methods , all of which are capable of detecting differences in gene expression between closely related types of cells . This section presents methods to ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector