Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-16
... centrifuge tube . Recover the cells by centrifugation at 1500g for 10 minutes at 4 ° C . Resuspend the cells in 5-10 volumes of ice - cold TBS and repeat the centrifugation . Resuspend the cells in TE ( pH 8.0 ) at a concentration of 5 ...
... centrifuge tube . Recover the cells by centrifugation at 1500g for 10 minutes at 4 ° C . Resuspend the cells in 5-10 volumes of ice - cold TBS and repeat the centrifugation . Resuspend the cells in TE ( pH 8.0 ) at a concentration of 5 ...
Page 14-23
... centrifuge , and take off the retentate cup . Add 0.75 ml of H2O to the sample reservoir , replace the retentate cup , and recentrifuge as described in step 4 . 6. Repeat step 5 twice more . At the end of the fourth centrifugation , the ...
... centrifuge , and take off the retentate cup . Add 0.75 ml of H2O to the sample reservoir , replace the retentate cup , and recentrifuge as described in step 4 . 6. Repeat step 5 twice more . At the end of the fourth centrifugation , the ...
Page 15-77
... centrifugation at 4000g for 5 minutes at room temperature . Avoid transferring material from the interface . 11. Transfer the aqueous phase from the final extraction to a glass centrifuge tube ( e.g. , a 30 - ml Corex tube ) . Measure ...
... centrifugation at 4000g for 5 minutes at room temperature . Avoid transferring material from the interface . 11. Transfer the aqueous phase from the final extraction to a glass centrifuge tube ( e.g. , a 30 - ml Corex tube ) . Measure ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector