Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxxii
... CHROMATOGRAPHY E.30 GEL - FILTRATION CHROMATOGRAPHY E.34 Preparation of Sephadex E.35 Column Chromatography E.36 SPUN - COLUMN CHROMATOGRAPHY E.37 PREPARATION OF DIALYSIS TUBING E.39 Appendix F : Subcloning FILLING RECESSED 3 ' TERMINI ...
... CHROMATOGRAPHY E.30 GEL - FILTRATION CHROMATOGRAPHY E.34 Preparation of Sephadex E.35 Column Chromatography E.36 SPUN - COLUMN CHROMATOGRAPHY E.37 PREPARATION OF DIALYSIS TUBING E.39 Appendix F : Subcloning FILLING RECESSED 3 ' TERMINI ...
Page 11-29
... Chromatography on a Silica Gel Isolate oligonucleotides from the filtered solution ( step 15 , page 11.28 ) either by reversed - phase chromatography on a silica gel as described below or by precipitation with ethanol . Precipitation ...
... Chromatography on a Silica Gel Isolate oligonucleotides from the filtered solution ( step 15 , page 11.28 ) either by reversed - phase chromatography on a silica gel as described below or by precipitation with ethanol . Precipitation ...
Page 1-7
... chromatography , 16.60–16.63 as reporter gene , 16.56 vectors , 16.57 4 - Chloro - 1 - naphthol , detection of antibody - horseradish peroxidase complexes , stock solution , 12.19 Chloroquine treatment following transfec- tion of ...
... chromatography , 16.60–16.63 as reporter gene , 16.56 vectors , 16.57 4 - Chloro - 1 - naphthol , detection of antibody - horseradish peroxidase complexes , stock solution , 12.19 Chloroquine treatment following transfec- tion of ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector