Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 53
Page xxiii
... Cleavage at G Residues 13.88 Cleavage at Purine Residues ( AG ) 13.90 Cleavage at Pyrimidine Residues ( C + T ) 13.91 Cleavage at C Residues 13.92 Cleavage at A and C Residues ( A > C ) 13.93 Treatment of Samples with Piperidine 13.94 ...
... Cleavage at G Residues 13.88 Cleavage at Purine Residues ( AG ) 13.90 Cleavage at Pyrimidine Residues ( C + T ) 13.91 Cleavage at C Residues 13.92 Cleavage at A and C Residues ( A > C ) 13.93 Treatment of Samples with Piperidine 13.94 ...
Page 13-88
... cleavage reactions are carried out on one day and the gels are run on the following day . However , with practice , it is possible to complete both cleavage and electrophoresis in a single working day , if a sequencing gel is prepared ...
... cleavage reactions are carried out on one day and the gels are run on the following day . However , with practice , it is possible to complete both cleavage and electrophoresis in a single working day , if a sequencing gel is prepared ...
Page 13-100
... cleavage reactions that do not normally share base specificity . Cleavage is consistent with base specificity every- where else in the sequencing pattern . 13. Difficulty in identifying the band corre- sponding to chemical cleavage of ...
... cleavage reactions that do not normally share base specificity . Cleavage is consistent with base specificity every- where else in the sequencing pattern . 13. Difficulty in identifying the band corre- sponding to chemical cleavage of ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector