Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 59
Page 8-24
... cleaved vector with a phosphatase before ligation to cDNA . If the double - stranded cDNA contains one or more recognition sites for the restriction enzyme , it will be cleaved and subsequently cloned as two or more DNA fragments ...
... cleaved vector with a phosphatase before ligation to cDNA . If the double - stranded cDNA contains one or more recognition sites for the restriction enzyme , it will be cleaved and subsequently cloned as two or more DNA fragments ...
Page 8-27
... cleaved . The linkers are therefore added only to one end of the double - stranded cDNA ( the end corresponding to the 3 ' terminus of the mRNA ) . The cDNA is then purified from excess unligated linkers , and the hairpin loop is cleaved ...
... cleaved . The linkers are therefore added only to one end of the double - stranded cDNA ( the end corresponding to the 3 ' terminus of the mRNA ) . The cDNA is then purified from excess unligated linkers , and the hairpin loop is cleaved ...
Page 13-100
... cleave riboadenylic acid , since ribonucleotides do not depurinate readily . The three other ribonucleotides should ... cleaved the 5 ' terminal nucleotide . Put some [ 32P ] phosphate in formamide- buffer - dyes solution , and run ...
... cleave riboadenylic acid , since ribonucleotides do not depurinate readily . The three other ribonucleotides should ... cleaved the 5 ' terminal nucleotide . Put some [ 32P ] phosphate in formamide- buffer - dyes solution , and run ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector