Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 29
Page 11-9
... codons . Regions rich in amino acids with six codon choices ( Leu , Ser , and Arg ) should be avoided . The number of codons that encode each amino acid are given below ( see also Figure 11.1 ) . 1 2 3 4 6 Met Phe Ile Val Leu Trp Tyr ...
... codons . Regions rich in amino acids with six codon choices ( Leu , Ser , and Arg ) should be avoided . The number of codons that encode each amino acid are given below ( see also Figure 11.1 ) . 1 2 3 4 6 Met Phe Ile Val Leu Trp Tyr ...
Page 11-11
... codons that differ only in the third position , so that at least two of the three nucleotides of each codon are guaranteed to be perfectly matched . Hybridization can therefore be carried out under comparatively stringent conditions ...
... codons that differ only in the third position , so that at least two of the three nucleotides of each codon are guaranteed to be perfectly matched . Hybridization can therefore be carried out under comparatively stringent conditions ...
Page 11-14
... codon choices that generate the sequence CpG between codons . This dinucleotide is significantly underrepresented in mammalian DNA ( Bird 1980 ) , and CpG occurs in intercodons at about one half of the expected frequency ( Lathe 1985 ) ...
... codon choices that generate the sequence CpG between codons . This dinucleotide is significantly underrepresented in mammalian DNA ( Bird 1980 ) , and CpG occurs in intercodons at about one half of the expected frequency ( Lathe 1985 ) ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector