Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 48
Page vii
... Colonies to Nitrocellulose Filters 1.92 Replicating Colonies onto Nitrocellulose Filters 1.93 METHOD 1 1.93 METHOD 2 1.96 Lysis of Colonies and Binding of DNA to Nitrocellulose Filters 1.98 METHOD 1 1.98 METHOD 2 1.100 Hybridization to ...
... Colonies to Nitrocellulose Filters 1.92 Replicating Colonies onto Nitrocellulose Filters 1.93 METHOD 1 1.93 METHOD 2 1.96 Lysis of Colonies and Binding of DNA to Nitrocellulose Filters 1.98 METHOD 1 1.98 METHOD 2 1.100 Hybridization to ...
Page 12-22
... colonies to regenerate . Generally , it is best to make only two replicas from a single master to avoid problems caused by smearing of the colonies . 10. To induce the expression of a gene cloned into a plasmid carrying the lac promoter ...
... colonies to regenerate . Generally , it is best to make only two replicas from a single master to avoid problems caused by smearing of the colonies . 10. To induce the expression of a gene cloned into a plasmid carrying the lac promoter ...
Page 15-72
... colonies almost certainly contain a mixture of wild - type and mutated molecules , it is necessary to carry out a second round of transformation and screening as outlined below . Note that simple restreaking of positive colonies does ...
... colonies almost certainly contain a mixture of wild - type and mutated molecules , it is necessary to carry out a second round of transformation and screening as outlined below . Note that simple restreaking of positive colonies does ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector