Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-18
... complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes : The absence of a com- plementary strand eliminates the possibility of forming nonproductive ...
... complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes : The absence of a com- plementary strand eliminates the possibility of forming nonproductive ...
Page 11-41
... complementary sequences at their 3 ' termini ( Ullrich et al . 1984a ) ( see Figure 11.2 ) . Both strands are extended , resulting in a duplex that may be considerably longer than either of the two original oligonucleotides ( see pages ...
... complementary sequences at their 3 ' termini ( Ullrich et al . 1984a ) ( see Figure 11.2 ) . Both strands are extended , resulting in a duplex that may be considerably longer than either of the two original oligonucleotides ( see pages ...
Page 15-97
... complementary to the opposite strand and that carries the desired mutations . These sets of complementary oligonucleotides are then mixed under conditions that will allow mismatched hybrids to form . If the complementary ...
... complementary to the opposite strand and that carries the desired mutations . These sets of complementary oligonucleotides are then mixed under conditions that will allow mismatched hybrids to form . If the complementary ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector