Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-49
... concentration of 500 μg / ml in hybridization solutions containing dextran sulfate ; in hybridization solutions that do not contain dextran sulfate , heparin is used at a concentration of 50 μg / ml ( Singh and Jones , 1984 ) ...
... concentration of 500 μg / ml in hybridization solutions containing dextran sulfate ; in hybridization solutions that do not contain dextran sulfate , heparin is used at a concentration of 50 μg / ml ( Singh and Jones , 1984 ) ...
Page 10-20
... concentration in excess of Km and one radiolabeled dNTP at a lower concentration , which is usually below Km Under these conditions , the proportion of radiolabel that is incorporated into DNA is very high , even though the rate of the ...
... concentration in excess of Km and one radiolabeled dNTP at a lower concentration , which is usually below Km Under these conditions , the proportion of radiolabel that is incorporated into DNA is very high , even though the rate of the ...
Page 14-30
... concentration of the sequences of interest in the original sample of nucleic acids . This is because the efficiency of the amplification process is greatly affected by small differences in any one of a number of variables . These ...
... concentration of the sequences of interest in the original sample of nucleic acids . This is because the efficiency of the amplification process is greatly affected by small differences in any one of a number of variables . These ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector