Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 87
Page 11-48
... containing either sodium or tetramethylammonium ions . Hybrids involving oligonucleotides 16 and 19 nucleotides in length melt over a smaller range of temperature in solvents containing TMACI than in solvents containing sodium salts ( 3 ...
... containing either sodium or tetramethylammonium ions . Hybrids involving oligonucleotides 16 and 19 nucleotides in length melt over a smaller range of temperature in solvents containing TMACI than in solvents containing sodium salts ( 3 ...
Page 12-38
... containing 0.2 % maltose , 50 μg / ml ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes ...
... containing 0.2 % maltose , 50 μg / ml ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes ...
Page 15-46
... containing LB agar supplemented with kanamycin and ampicillin , each at a concentration of 100 μg / ml . Incubate the plates for 18-24 hours at 37 ° C . 13. At the end of the incubation , add to each plate approximately 5 ml of LB ...
... containing LB agar supplemented with kanamycin and ampicillin , each at a concentration of 100 μg / ml . Incubate the plates for 18-24 hours at 37 ° C . 13. At the end of the incubation , add to each plate approximately 5 ml of LB ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector