Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-8
... dNTPs ( if needed ) [ a - 32P ] dNTP H2O to 21.5 μl Chill the mixture to 0 ° C . 2.5 με 0.5 με 20 nmoles of each 16 pmoles For unlabeled dNTPs , add 1 μl of a 20 mM solution of each unlabeled dNTP ; usually , 20 nmoles of three unlabeled ...
... dNTPs ( if needed ) [ a - 32P ] dNTP H2O to 21.5 μl Chill the mixture to 0 ° C . 2.5 με 0.5 με 20 nmoles of each 16 pmoles For unlabeled dNTPs , add 1 μl of a 20 mM solution of each unlabeled dNTP ; usually , 20 nmoles of three unlabeled ...
Page 10-9
... dNTP . Most commercial suppliers sell [ a - 32P ] dNTPs in a concentrated aqueous solution , which can be added directly to the nick - translation reaction . The specific activities range from 400 to > 3000 Ci / mmole . If [ a - 32P ] dNTPs ...
... dNTP . Most commercial suppliers sell [ a - 32P ] dNTPs in a concentrated aqueous solution , which can be added directly to the nick - translation reaction . The specific activities range from 400 to > 3000 Ci / mmole . If [ a - 32P ] dNTPs ...
Page 13-59
... dNTPs and a lower concentration of one of these dNTPs . The names of these three working solutions identify the particular dNTP that is present in low concentration . For example , the working solution of dCTP contains a lower ...
... dNTPs and a lower concentration of one of these dNTPs . The names of these three working solutions identify the particular dNTP that is present in low concentration . For example , the working solution of dCTP contains a lower ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector