Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxiv
... Deletions and Insertions 15.3 SIMPLE DELETIONS OR INSERTIONS 15.3 SYSTEMATIC DELETIONS AND INSERTIONS Linker - insertion Mutagenesis 15.5 15.5 GENERATION OF LINKER - INSERTION MUTANTS WITH FREQUENTLY CUTTING RESTRICTION ENZYMES 15.8 ...
... Deletions and Insertions 15.3 SIMPLE DELETIONS OR INSERTIONS 15.3 SYSTEMATIC DELETIONS AND INSERTIONS Linker - insertion Mutagenesis 15.5 15.5 GENERATION OF LINKER - INSERTION MUTANTS WITH FREQUENTLY CUTTING RESTRICTION ENZYMES 15.8 ...
Page 13-38
... deletions , but also groups of deletions whose endpoints are tightly clustered . ( 2 ) The method is not restricted to any particular vector . ( 3 ) The entire series of enzymatic reactions ( exonuclease digestion , treatment with ...
... deletions , but also groups of deletions whose endpoints are tightly clustered . ( 2 ) The method is not restricted to any particular vector . ( 3 ) The entire series of enzymatic reactions ( exonuclease digestion , treatment with ...
Page 15-3
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Generation of Deletions and Insertions SIMPLE DELETIONS OR INSERTIONS The simplest mutations to make are deletions of segments of foreign DNA sequences cloned in plasmid vectors . If the ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Generation of Deletions and Insertions SIMPLE DELETIONS OR INSERTIONS The simplest mutations to make are deletions of segments of foreign DNA sequences cloned in plasmid vectors . If the ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector