Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 78
Page 8-73
... determine the amount of cDNA required to yield a population of bac- teriophages that contains 5 % recombinants . In this case , the cDNA is titrated to determine the amount that yields a tenfold stimulation of total plaques over the ...
... determine the amount of cDNA required to yield a population of bac- teriophages that contains 5 % recombinants . In this case , the cDNA is titrated to determine the amount that yields a tenfold stimulation of total plaques over the ...
Page 13-14
... determine where these subclones map in the target DNA or which strand of DNA is being sequenced . Instead , the accumulated data are stored and finally arranged in order by a computer ( Staden 1986 ) . This approach , which was ...
... determine where these subclones map in the target DNA or which strand of DNA is being sequenced . Instead , the accumulated data are stored and finally arranged in order by a computer ( Staden 1986 ) . This approach , which was ...
Page 15-76
... Determine the relative titer of the bacteriophage suspension on E. coli strain CJ236 ( dut ̄ ung ̄ F ' ) and a strain such as JM109 or TG1 ( see Chapter 4 , page 4.14 ) . The titer on strain CJ236 should be four to five orders of ...
... Determine the relative titer of the bacteriophage suspension on E. coli strain CJ236 ( dut ̄ ung ̄ F ' ) and a strain such as JM109 or TG1 ( see Chapter 4 , page 4.14 ) . The titer on strain CJ236 should be four to five orders of ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector