Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 75
Page 9-24
... digestion of linkers ) to generate cohesive termini compatible with those of the vectors used to generate genomic DNA libraries ( Maniatis et al . 1978 ) . On the other hand , partial digestion with restriction enzymes that recognize ...
... digestion of linkers ) to generate cohesive termini compatible with those of the vectors used to generate genomic DNA libraries ( Maniatis et al . 1978 ) . On the other hand , partial digestion with restriction enzymes that recognize ...
Page 9-32
... digestion of high - molecular - weight DNA is unevenness of digestion caused by variations in the local concentrations of DNA . Clumps of DNA are relatively inaccessible to restriction enzymes and can be digested only from the outside ...
... digestion of high - molecular - weight DNA is unevenness of digestion caused by variations in the local concentrations of DNA . Clumps of DNA are relatively inaccessible to restriction enzymes and can be digested only from the outside ...
Page 15-27
... digestion , remove an aliquot of DNAase I from storage at -70 ° C and dilute it 1 : 1000 into ice - cold 1 × DNAase / Mn ++ digestion buffer . The concentration of the diluted enzyme should be 10 ng / μl . ++ 10 × DNAase / Mn digestion ...
... digestion , remove an aliquot of DNAase I from storage at -70 ° C and dilute it 1 : 1000 into ice - cold 1 × DNAase / Mn ++ digestion buffer . The concentration of the diluted enzyme should be 10 ng / μl . ++ 10 × DNAase / Mn digestion ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector