Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-18
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
Page 14-28
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
Page 15-20
... double- stranded DNA is given by the equation : dM = -2VMn dt [ Km + ( S ) , ] t n m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , Vm = maximum reaction velocity ( expressed as moles of nu ...
... double- stranded DNA is given by the equation : dM = -2VMn dt [ Km + ( S ) , ] t n m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , Vm = maximum reaction velocity ( expressed as moles of nu ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector