Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-23
... efficiency of cloning , the number of homopolymeric residues on the plasmid and the cDNA should be approximately equal , with approxi- mately 100 dA / dT residues or 20 dC / dG residues being added to each end of the DNAs ( Peacock et ...
... efficiency of cloning , the number of homopolymeric residues on the plasmid and the cDNA should be approximately equal , with approxi- mately 100 dA / dT residues or 20 dC / dG residues being added to each end of the DNAs ( Peacock et ...
Page 14-30
... efficiency of the amplification process is greatly affected by small differences in any one of a number of variables . These include ( 1 ) the quality and concentrations of the reagents used ( Taq DNA polymerase , dNTPs ...
... efficiency of the amplification process is greatly affected by small differences in any one of a number of variables . These include ( 1 ) the quality and concentrations of the reagents used ( Taq DNA polymerase , dNTPs ...
Page 15-61
... efficiency with which it will be generated . For example , large deletions ( i.e. , deletions of several hundred nucleotides ) are generated with approximately 50 - fold lower efficiency than mutations involving only local changes in ...
... efficiency with which it will be generated . For example , large deletions ( i.e. , deletions of several hundred nucleotides ) are generated with approximately 50 - fold lower efficiency than mutations involving only local changes in ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector