Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiii
... Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 ... ELECTROPHORESIS BUFFER 6.6 Apparatuses Used for Agarose Gel Electrophoresis 6.8 PREPARATION AND EXAMINATION OF AGAROSE ...
... Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 ... ELECTROPHORESIS BUFFER 6.6 Apparatuses Used for Agarose Gel Electrophoresis 6.8 PREPARATION AND EXAMINATION OF AGAROSE ...
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... electrophoresis precautions for laboratory safety , 6.39 , B.9 properties , 6.36 , 18.49 stock solutions , 6.39 ... electrophoresis alkaline analysis of first- and second - strand cDNA synthesis , 8.5 analysis of single - stranded DNA ...
... electrophoresis precautions for laboratory safety , 6.39 , B.9 properties , 6.36 , 18.49 stock solutions , 6.39 ... electrophoresis alkaline analysis of first- and second - strand cDNA synthesis , 8.5 analysis of single - stranded DNA ...
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... electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning vectors , 4.2 , 4.7 problems encountered in use ...
... electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning vectors , 4.2 , 4.7 problems encountered in use ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector