Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-32
... encoding any subunit of a multisubunit protein ( Lübbert et al . 1987 ) . The principle of the method is shown in Figure 8.11 . A population of mRNA molecules that includes species encoding all of the subunits of a multifunctional ...
... encoding any subunit of a multisubunit protein ( Lübbert et al . 1987 ) . The principle of the method is shown in Figure 8.11 . A population of mRNA molecules that includes species encoding all of the subunits of a multifunctional ...
Page 8-33
... encoding the subunits of a multisubunit protein of interest Microinject into Xenopus oocytes . Score for activity of multisubunit protein of interest . Hybridize in solution to pools of recombinant cDNAs constructed in single - stranded ...
... encoding the subunits of a multisubunit protein of interest Microinject into Xenopus oocytes . Score for activity of multisubunit protein of interest . Hybridize in solution to pools of recombinant cDNAs constructed in single - stranded ...
Page 12-42
... encoding yeast DNA polymerase I. Cell 43 : 369 . Kadonaga , J.T. and R. Tjian . 1986. Af- finity purification of sequence - specific DNA binding proteins . Proc . Natl . Acad . Sci . 83 : 5889 . Kadonaga , J.T. , K.R. Carner , F.R. Mas ...
... encoding yeast DNA polymerase I. Cell 43 : 369 . Kadonaga , J.T. and R. Tjian . 1986. Af- finity purification of sequence - specific DNA binding proteins . Proc . Natl . Acad . Sci . 83 : 5889 . Kadonaga , J.T. , K.R. Carner , F.R. Mas ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector