Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 86
Page 8-55
... ethanol bath ) for 15 minutes or to store it for more prolonged periods at -20 ° C before centrifugation . In our hands , these procedures do not improve the efficiency with which DNA is precipitated by ethanol . • Save the ethanol ...
... ethanol bath ) for 15 minutes or to store it for more prolonged periods at -20 ° C before centrifugation . In our hands , these procedures do not improve the efficiency with which DNA is precipitated by ethanol . • Save the ethanol ...
Page 9-22
... ethanol at room temperature into each of a series of disposable 50 - ml polypropylene centrifuge tubes . Using wide - bore pi- pettes , carefully layer the cell suspensions under the ethanol . 5. Recover the DNA from each tube by slowly ...
... ethanol at room temperature into each of a series of disposable 50 - ml polypropylene centrifuge tubes . Using wide - bore pi- pettes , carefully layer the cell suspensions under the ethanol . 5. Recover the DNA from each tube by slowly ...
Page 13-98
... ethanol precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
... ethanol precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector