Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 48
Page vi
... Ethidium Bromide Gradients CONTINUOUS GRADIENTS 1.42 DISCONTINUOUS GRADIENTS 1.44 1.42 Removal of Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ...
... Ethidium Bromide Gradients CONTINUOUS GRADIENTS 1.42 DISCONTINUOUS GRADIENTS 1.44 1.42 Removal of Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ...
Page xiii
... Ethidium Bromide Solutions 6.16 DECONTAMINATION OF CONCENTRATED SOLUTIONS OF ETHIDIUM BROMIDE 6.16 DECONTAMINATION OF DILUTE SOLUTIONS OF ETHIDIUM BROMIDE 6.17 Photography 6.19 Alkaline Agarose Gels 6.20 RECOVERY AND PURIFICATION OF DNA ...
... Ethidium Bromide Solutions 6.16 DECONTAMINATION OF CONCENTRATED SOLUTIONS OF ETHIDIUM BROMIDE 6.16 DECONTAMINATION OF DILUTE SOLUTIONS OF ETHIDIUM BROMIDE 6.17 Photography 6.19 Alkaline Agarose Gels 6.20 RECOVERY AND PURIFICATION OF DNA ...
Page 9-33
... ethidium bromide ( ~ 200 ng ) should be applied to the gel . Occasionally , problems arise during loading of the gel because the DNA solution will not sink to the bottom of the well . This occurs when very - high - molecular - weight ...
... ethidium bromide ( ~ 200 ng ) should be applied to the gel . Occasionally , problems arise during loading of the gel because the DNA solution will not sink to the bottom of the well . This occurs when very - high - molecular - weight ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector