Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-9
... eukaryotic DNA in bacteriophage A vectors are shown in Figure 9.1 and are summarized as follows : 1. The DNA of a substitution vector of bacteriophage that will accept 20-24 - kb segments of foreign DNA is digested with a restriction ...
... eukaryotic DNA in bacteriophage A vectors are shown in Figure 9.1 and are summarized as follows : 1. The DNA of a substitution vector of bacteriophage that will accept 20-24 - kb segments of foreign DNA is digested with a restriction ...
Page 9-10
... Eukaryotic genomic DNA COS | Digest with BamHI COS COS internal fragments Remove internal fragments not essential to ... eukaryotic DNA . ( Left ) Preparation of the vector DNA fragments ; ( right ) preparation of eukaryotic DNA ...
... Eukaryotic genomic DNA COS | Digest with BamHI COS COS internal fragments Remove internal fragments not essential to ... eukaryotic DNA . ( Left ) Preparation of the vector DNA fragments ; ( right ) preparation of eukaryotic DNA ...
Page 9-24
... Eukaryotic DNA with Restriction Enzymes The only method by which DNA can be fragmented in truly random fashion , irrespective of its base composition ... Eukaryotic Genomic DNA Partial Digestion of High-molecular-weight Eukaryotic DNA with.
... Eukaryotic DNA with Restriction Enzymes The only method by which DNA can be fragmented in truly random fashion , irrespective of its base composition ... Eukaryotic Genomic DNA Partial Digestion of High-molecular-weight Eukaryotic DNA with.
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector