Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 43
Page 13-38
... exonuclease III is by far the best for several reasons . ( 1 ) The exonucleolytic reaction proceeds at a remarkably uniform and predictable rate . This means that it is possible to isolate not only nested deletions , but also groups of ...
... exonuclease III is by far the best for several reasons . ( 1 ) The exonucleolytic reaction proceeds at a remarkably uniform and predictable rate . This means that it is possible to isolate not only nested deletions , but also groups of ...
Page 13-39
... Exonuclease III 1. Digest 10 μg of recombinant bacteriophage M13 replicative form DNA or recombinant phagemid DNA with two restriction enzymes that cleave in the polycloning site between the primer - binding site of the vector and the ...
... Exonuclease III 1. Digest 10 μg of recombinant bacteriophage M13 replicative form DNA or recombinant phagemid DNA with two restriction enzymes that cleave in the polycloning site between the primer - binding site of the vector and the ...
Page 15-38
... exonuclease III / depurination buffer . 1x Exonuclease III / depurination buffer 66 mм Tris Cl ( pH 8.0 ) 125 mM NaCl 5 mM CaCl2 10 mм dithiothreitol CaCl , is used rather than MgCl , to suppress the exonucleolytic activity of exonuclease ...
... exonuclease III / depurination buffer . 1x Exonuclease III / depurination buffer 66 mм Tris Cl ( pH 8.0 ) 125 mM NaCl 5 mM CaCl2 10 mм dithiothreitol CaCl , is used rather than MgCl , to suppress the exonucleolytic activity of exonuclease ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector