Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiv
... Extraction with Strong Denaturants 7.18 EXTRACTION OF RNA WITH GUANIDINIUM THIOCYANATE FOLLOWED BY CENTRIFUGATION IN CESIUM CHLORIDE SOLUTIONS 7.19 EXTRACTION OF RNA WITH GUANIDINE HCI AND ORGANIC SOLVENTS 7.23 + SELECTION OF POLY ( A ) ...
... Extraction with Strong Denaturants 7.18 EXTRACTION OF RNA WITH GUANIDINIUM THIOCYANATE FOLLOWED BY CENTRIFUGATION IN CESIUM CHLORIDE SOLUTIONS 7.19 EXTRACTION OF RNA WITH GUANIDINE HCI AND ORGANIC SOLVENTS 7.23 + SELECTION OF POLY ( A ) ...
Page 9-16
... extraction buffer for each milliliter of cell suspension . Incubate the solution for 1 hour at 37 ° C , and then proceed to step 2 . Extraction buffer 10 mM Tris Cl ( pH 8.0 ) 0.1 м EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 % SDS ...
... extraction buffer for each milliliter of cell suspension . Incubate the solution for 1 hour at 37 ° C , and then proceed to step 2 . Extraction buffer 10 mM Tris Cl ( pH 8.0 ) 0.1 м EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 % SDS ...
Page 9-17
... extraction buffer ( see above ) in a beaker . Allow the powder to spread over the surface of the extraction buffer , and then shake the beaker to submerge the material . When all of the material is in solution , transfer the solution to ...
... extraction buffer ( see above ) in a beaker . Allow the powder to spread over the surface of the extraction buffer , and then shake the beaker to submerge the material . When all of the material is in solution , transfer the solution to ...
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agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector