Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 53
Page 9-49
... concentrations of SDS , which will cause the milk proteins to precipitate . If background hybridization is a problem , NP - 40 may be added to the hybridization solution to a final concentration of 1 % . BLOTTO should not be used as a ...
... concentrations of SDS , which will cause the milk proteins to precipitate . If background hybridization is a problem , NP - 40 may be added to the hybridization solution to a final concentration of 1 % . BLOTTO should not be used as a ...
Page 13-84
... final concentration of 0.15 M , and purify the sonicated DNA by three sequential extractions with phenol : chloroform . Concentrate the DNA by precipi- tation with 2 volumes of ethanol ( 15 minutes at room temperature ) and ...
... final concentration of 0.15 M , and purify the sonicated DNA by three sequential extractions with phenol : chloroform . Concentrate the DNA by precipi- tation with 2 volumes of ethanol ( 15 minutes at room temperature ) and ...
Page 14-20
... final concentration 100 μg / ml ) may be substituted for gelatin in the reverse transcription reaction . The amount of MgCl2 in the amplification buffer needs to be supplemented with additional MgCl2 in the reverse transcriptase ...
... final concentration 100 μg / ml ) may be substituted for gelatin in the reverse transcription reaction . The amount of MgCl2 in the amplification buffer needs to be supplemented with additional MgCl2 in the reverse transcriptase ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector