Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
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Page 9-7
... DNA The first generation of bacteriophage vectors , such as Charon 4A ( see Chapter 2 , page 2.19 ) and Agt - WES ( see Maniatis et al . 1982 for map ) , which were developed in the late 1970s , could accept segments of foreign DNA ...
... DNA The first generation of bacteriophage vectors , such as Charon 4A ( see Chapter 2 , page 2.19 ) and Agt - WES ( see Maniatis et al . 1982 for map ) , which were developed in the late 1970s , could accept segments of foreign DNA ...
Page 10-29
... foreign DNA sequences can usually be inserted by directional cloning no further than 30-40 nucleotides downstream from the position at which RNA synthesis is initiated in vitro . In many cases , the polycloning site also provides a ...
... foreign DNA sequences can usually be inserted by directional cloning no further than 30-40 nucleotides downstream from the position at which RNA synthesis is initiated in vitro . In many cases , the polycloning site also provides a ...
Page 15-3
... foreign DNA sequences cloned in plasmid vectors . If the restriction map or , better yet , the complete nucleotide sequence of the foreign DNA is known , it may be possible to construct a set of deletion mutants by digestion with the ...
... foreign DNA sequences cloned in plasmid vectors . If the restriction map or , better yet , the complete nucleotide sequence of the foreign DNA is known , it may be possible to construct a set of deletion mutants by digestion with the ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector