Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiii
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 ... GELS 6.49 7 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xiii.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 ... GELS 6.49 7 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xiii.
Page xiv
... Gel Electrophoresis 6.52 Preparation of DNA for Pulsed - field Gel Electrophoresis 6.53 ISOLATION OF INTACT DNA FROM MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.55 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 ...
... Gel Electrophoresis 6.52 Preparation of DNA for Pulsed - field Gel Electrophoresis 6.53 ISOLATION OF INTACT DNA FROM MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.55 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 ...
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... electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous ... gels , 7.44 formamide / EDTA / XC / BPB gel - loading 1.16 Index.
... electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous ... gels , 7.44 formamide / EDTA / XC / BPB gel - loading 1.16 Index.
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector