Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-37
... gene product is controlled by the host hflA gene and the cIII gene product . Mutation of the hflA gene enhances the stability of the cII gene product , resulting in an increased production of the cl gene product and a high frequency of ...
... gene product is controlled by the host hflA gene and the cIII gene product . Mutation of the hflA gene enhances the stability of the cII gene product , resulting in an increased production of the cl gene product and a high frequency of ...
Page 11-14
... gene families occasionally display marked bias for or against certain codons ( e.g. , histone genes show a marked preference for codons enriched in A and T residues ) . If there is any evidence for such a bias in the gene of interest ...
... gene families occasionally display marked bias for or against certain codons ( e.g. , histone genes show a marked preference for codons enriched in A and T residues ) . If there is any evidence for such a bias in the gene of interest ...
Page 12-6
... gene of interest : the smaller the gene , the smaller the genomic DNA fragments . Ideally , the size of the fragments should be approximately one half the predicted size of the gene . This reduces the chance that the coding sequences of ...
... gene of interest : the smaller the gene , the smaller the genomic DNA fragments . Ideally , the size of the fragments should be approximately one half the predicted size of the gene . This reduces the chance that the coding sequences of ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector