Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 95
Page 9-2
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from ... genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by complete digestion of genomic ...
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from ... genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by complete digestion of genomic ...
Page 9-31
... genomic DNA is usually accom- plished by the transfer techniques described by Southern ( 1975 ) . Genomic DNA is digested with one or more restriction enzymes , and the resulting fragments are separated according to size by ...
... genomic DNA is usually accom- plished by the transfer techniques described by Southern ( 1975 ) . Genomic DNA is digested with one or more restriction enzymes , and the resulting fragments are separated according to size by ...
Page 12-6
... genomes contain few introns , there is little to be gained by constructing cDNA libraries in these vectors . Instead , randomly sheared genomic DNA can be used to create a library ( for details , see Chapter 9 ) in which all coding ...
... genomes contain few introns , there is little to be gained by constructing cDNA libraries in these vectors . Instead , randomly sheared genomic DNA can be used to create a library ( for details , see Chapter 9 ) in which all coding ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector