Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 32
Page 8-74
... hours at 37 ° C or 42 ° C . Plaque formation with Agt10 : Grow E. coli strains C600 and BNN102 in NZCYM medium containing 0.2 % maltose . ( BNN102 grows much more slowly than C600 . ) Prepare stocks of plating bacteria of both strains ...
... hours at 37 ° C or 42 ° C . Plaque formation with Agt10 : Grow E. coli strains C600 and BNN102 in NZCYM medium containing 0.2 % maltose . ( BNN102 grows much more slowly than C600 . ) Prepare stocks of plating bacteria of both strains ...
Page 8-78
... 37 ° C . Then plate on a petri dish containing NZCYM agar , and incubate for 8-10 hours at 37 ° C . In some cases , the use of large volumes of packaging mixture leads to a reduction in plating efficiency . This should be checked before ...
... 37 ° C . Then plate on a petri dish containing NZCYM agar , and incubate for 8-10 hours at 37 ° C . In some cases , the use of large volumes of packaging mixture leads to a reduction in plating efficiency . This should be checked before ...
Page 12-18
... C or 5 minutes at -20 ° C . c . Remove the filters and immediately immerse them in TNT . d . Overlay each of the plates with a second filter impregnated with IPTG . e . Incubate the plates for a further 4-6 hours at 37 ° C . f ...
... C or 5 minutes at -20 ° C . c . Remove the filters and immediately immerse them in TNT . d . Overlay each of the plates with a second filter impregnated with IPTG . e . Incubate the plates for a further 4-6 hours at 37 ° C . f ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector